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Abstract The bacterial protein quality control (PQC) network comprises a set of genes that promote proteostasis (proteome homeostasis) through proper protein folding and function via chaperones, proteases, and protein translational machinery. It participates in vital cellular processes and influences organismal development and evolution. In this review, we examine the mechanistic bases for how the bacterial PQC network influences molecular evolution. We discuss the relevance of PQC components to contemporary issues in evolutionary biology including epistasis, evolvability, and the navigability of protein space. We examine other areas where proteostasis affects aspects of evolution and physiology, including host-parasite interactions. More generally, we demonstrate that the study of bacterial systems can aid in broader efforts to understand the relationship between genotype and phenotype across the biosphere. 

BackgroundHealthy articular cartilage presents structural gradients defined by distinct zonal patterns through the thickness, which may be disrupted in the pathogenesis of several disorders. Analysis of textural patterns using quantitative MRI data may identify structural gradients of healthy or degenerating tissue that correlate with early osteoarthritis (OA). PurposeTo quantify spatial gradients and patterns in MRI data, and to probe new candidate biomarkers for early severity of OA. Study TypeRetrospective study. SubjectsFourteen volunteers receiving total knee replacement surgery (eight males/two females/four unknown, average age ± standard deviation: 68.1 ± 9.6 years) and 10 patients from the OA Initiative (OAI) with radiographic OA onset (two males/eight females, average age ± standard deviation: 57.7 ± 9.4 years; initial Kellgren‐Lawrence [KL] grade: 0; final KL grade: 3 over the 10‐year study). Field Strength/Sequence3.0‐T and 14.1‐T, biomechanics‐based displacement‐encoded imaging, fast spin echo, multi‐slice multi‐echoT2mapping. AssessmentWe studied structure and strain in cartilage explants from volunteers receiving total knee replacement, or structure in cartilage of OAI patients with progressive OA. We calculated spatial gradients of quantitative MRI measures (eg, T2) normal to the cartilage surface to enhance zonal variations. We compared gradient values against histologically OA severity, conventional relaxometry, and/or KL grades. Statistical TestsMultiparametric linear regression for evaluation of the relationship between residuals of the mixed effects models and histologically determined OA severity scoring, with a significance threshold atα = 0.05. ResultsGradients of individual relaxometry and biomechanics measures significantly correlated with OA severity, outperforming conventional relaxometry and strain metrics. In human explants, analysis of spatial gradients provided the strongest relationship to OA severity (R² = 0.627). Spatial gradients of T2 from OAI data identified variations in radiographic (KL Grade 2) OA severity in single subjects, while conventional T2 alone did not. Data ConclusionSpatial gradients of quantitative MRI data may improve the predictive power of noninvasive imaging for early‐stage degeneration. Evidence Level1 Technical EfficacyStage 1 

Abstract The Transiting Exoplanet Survey Satellite (TESS) has surveyed nearly the entire sky in full-frame image mode with a time resolution of 200 s to 30 minutes and a temporal baseline of at least 27 days. In addition to the primary goal of discovering new exoplanets, TESS is exceptionally capable at detecting variable stars, and in particular short-period eclipsing binaries, which are relatively common, making up a few percent of all stars, and represent powerful astrophysical laboratories for deep investigations of stellar formation and evolution. We combed Sectors 1–82 of the TESS full-frame image data searching for eclipsing binary stars using a neural network that identified ∼1.2 million stars with eclipse-like features. Of these, we have performed an in-depth analysis on ∼60,000 targets using automated methods and manual inspection by citizen scientists. Here we present a catalog of 10,001 uniformly vetted and validated eclipsing binary stars that passed all our ephemeris and photocenter tests, as well as complementary visual inspection. Of these, 7936 are new eclipsing binaries while the remaining 2065 are known systems for which we update the published ephemerides. We outline the detection and analysis of the targets, discuss the properties of the sample, and highlight potentially interesting systems. Finally, we also provide a list of ∼900,000 unvetted and unvalidated targets for which the neural network found eclipse-like features with a score higher than 0.9, and for which there are no known eclipsing binaries within a sky-projected separation of a TESS pixel (≈21″). 

Abstract Transdisciplinary research knits together knowledge from diverse epistemic communities in addressing social-environmental challenges, such as biodiversity loss, climate crises, food insecurity, and public health. This article reflects on the roles of philosophy of science in transdisciplinary research while focusing on Indigenous and other subjugated forms of knowledge. We offer a critical assessment of demarcationist approaches in philosophy of science and outline a constructive alternative of transdisciplinary philosophy of science. While a focus on demarcation obscures the complex relations between epistemic communities, transdisciplinary philosophy of science provides resources for meeting epistemic and political challenges of collaborative knowledge production. 

Abstract The Differential Speckle Survey Instrument (DSSI) was relocated to the Astrophysical Research Consortium 3.5 m telescope at Apache Point Observatory (APO) in early 2022. Here we present results from the first year of observations along with an updated instrument description for DSSI at APO, including a detailed description of a new internal slit mask assembly used to measure the instrument plate scale from first principles. Astrometric precision for DSSI at APO during this time was measured to be 2.06 ± 0.11 mas, with a photometric precision of 0.14 ± 0.04 mag. Results of 40 resolved binary systems are reported, including two that were previously unknown to be binaries: HIP 7535 and HIP 9603. We also present updated orbital fits for two systems: HIP 93903 and HIP 100714. Finally, we report updated or confirmed dispositions for five Kepler Objects of Interest (KOIs) that were previously explored in Colton et al., using speckle imaging to discern common proper motions pairs from line of sight companions: KOI-270, KOI-959, KOI-1613, KOI-1962, and KOI-3214AB.